拓撲異構酶Ⅱ(TOP2)重組蛋白
Recombinant Topoisomerase II (TOP2)
DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
- 編號RPA792Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞核
- 預測分子量32.1kDa
- 實際分子量40kDa(差異分析請參閱說明書)
- 片段與標簽Gly1079~Ser1328 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點6.1
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1104 ¥ 2760 ¥ 5520 ¥ 16560 ¥ 41400
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序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA792Ra01 | 拓撲異構酶Ⅱ(TOP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA792Ra01 | 拓撲異構酶Ⅱ(TOP2)多克隆抗體 | WB; IHC; ICC; IP. |
MAA792Ra21 | 拓撲異構酶Ⅱ(TOP2)單克隆抗體 | WB; IHC; ICC; IP. |
SEA792Ra | 拓撲異構酶Ⅱ(TOP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA792Ra | 拓撲異構酶Ⅱ(TOP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II [pubmed:27852319] |
Bioorganic & Medicinal Chemistry | Synthesis of pharmacologically important naphthoquinones and anticancer activity of 2-benzyllawsone through DNA topoisomerase-II inhibition. [pubmed:28094224] |
European Journal of Medicinal Chemistry | Antiproliferative activity of diarylnaphthylpyrrolidine derivative via dual target inhibition [Pubmed: 31884407] |
Int J Mol Sci | 2-{5-[(Z,2Z)-2-Chloro-3-(4-nitrophenyl)-2-propenylidene]-4-oxo-2-thioxothiazolidin-3-yl}-3-methylbutanoic Acid as a Potential Anti-Breast Cancer Molecule [Pubmed:35456915] |
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