綠色熒光蛋白(GFP)重組抗體
Recombinant Antibody to Green Fluorescent Protein (GFP)
- 編號(hào)RAD025Ge71
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 來(lái)源重組單抗制備
- 宿主CHO
- 效價(jià)(克隆號(hào))-
- Ig亞型 IgG
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物生物素
- 免疫原 EPD025Ge51-綠色熒光蛋白(GFP)真核蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Pan-species (General，通用)
- 應(yīng)用WB; IF; ICC; IHC; IP; FCM.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 1346 ¥ 3142 ¥ 4488 ¥ 11220 ¥ 44880
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Briefly, biotin labelled GFP Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GFP and GFP Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.0187 ug/ml.
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GFP?was?immunoprecipitated?293F?whole?cell?extract (Expressing?GFP) with?2?μg?of?RAD025Ge71?and?20?μl?of?Nickel?pillar?magnetic?beads?( ).?
Western?blot was perfomed on the immunoprecipitate using?0.05μg/ml?MAD025Ge21 and 0.2μg/mL?SAA544Mu19.
Lane1 (input):?293F?whole?cell?lysate.
Lane2 ( ):?RAD025Ge71?in?293F?whole?cell?lysate.
Lane3 (-):?No?antibody?was?added?to?the?293F?whole?cell?lysate.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)GFP的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別GFP。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPD025Ge51 | 綠色熒光蛋白(GFP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD025Ge01 | 綠色熒光蛋白(GFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD025Ge02 | 綠色熒光蛋白(GFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD025Ge07 | 綠色熒光蛋白(GFP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD025Ge02 | 綠色熒光蛋白(GFP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD025Ge51 | 綠色熒光蛋白(GFP)多克隆抗體 | WB; IHC; ICC; IP. |
| MAD025Ge21 | 綠色熒光蛋白(GFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD025Ge22 | 綠色熒光蛋白(GFP)單克隆抗體 | WB; IHC; ICC; IP. |
| RAD025Ge71 | 綠色熒光蛋白(GFP)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAD025Ge51 | 綠色熒光蛋白(GFP)單克隆抗體 | WB; IHC; ICC; IP. |
| SED025Ge | 綠色熒光蛋白(GFP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AED025Ge | 抗綠色熒光蛋白抗體(Anti-GFP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMD025Ge | 綠色熒光蛋白(GFP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSD025Ge01 | 綠色熒光蛋白(GFP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system [] | |
| FASEB J | CircPrkcsh, a circular RNA, contributes to the polarization of microglia towards the M1 phenotype induced by spinal cord injury and acts via the JNK/p38 MAPK?… [34751973] |
| Microbiol Spectr | Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells [Pubmed:35604158] |
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