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血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Von Willebrand Factor (vWF) ,etc. by FLIA (Flow Luminescence Immunoassay)

F8VWF; VWD; von Willebrand antigen 2

（注：單次混測多因子不超過8個指標 ）

  • 血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • 血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 84-98 91

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 96-103% 78-102% 95-103%
EDTA plasma(n=5) 78-92% 85-103% 88-99% 87-97%
heparin plasma(n=5) 93-101% 89-104% 83-93% 87-101%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

相關產品

編號 適用物種：Sus scrofa; Porcine (Pig，豬) 應用(僅供研究使用，不用于臨床診斷！)
RPA833Po01 血管性血友病因子(vWF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAA833Po01 血管性血友病因子(vWF)多克隆抗體 WB; IHC; ICC; IP.
PAA833Po08 血管性血友病因子(vWF)多克隆抗體 IHC; ICC; IP.
LAA833Po81 血管性血友病因子(vWF)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
MAA833Po21 血管性血友病因子(vWF)單克隆抗體 WB; IHC; ICC; IP.
CEA833Po 血管性血友病因子(vWF)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA833Po 血管性血友病因子(vWF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

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