低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標 ）
- 編號LMB107Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.39-400ng/mL
- 靈敏度最小可檢測劑量小于等于0.13 ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-102 | 96 |
| EDTA plasma(n=5) | 79-90 | 83 |
| heparin plasma(n=5) | 85-103 | 98 |
| sodium citrate plasma(n=5) | 91-99 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 79-95% | 90-99% | 95-103% |
| EDTA plasma(n=5) | 99-105% | 93-105% | 94-101% | 78-104% |
| heparin plasma(n=5) | 83-91% | 91-98% | 96-105% | 81-89% |
| sodium citrate plasma(n=5) | 93-101% | 95-102% | 82-93% | 80-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SEB107Ra | 低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB107Ra | 低密度脂蛋白(LDL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. [NCBI: PMC3896367] |
| PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL [Pubmed:24465540] |
| J Lipid Res | PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in mice [PubMed: 26323289] |
| Neuroscience | 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain [PubMed: 25987203] |
| The Journal of Nutritional Biochemistry | Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice [Pubmed:27142749] |
| ?Journal of Functional Foods | Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice [S1756464617300555] |
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| Journal of Surgical Arts/Cerrahi Sanatlar Dergisi | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Hiperlipidemili ve … [163] |
| Journal of Functional Foods | Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice [10.1016:j.jff.2017.01.044] |
| Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients [pubmed:28954218] |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189] |
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| American Journal of Translational?Research | Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarction [Pubmed: 30662674] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| Diabetes Metab Syndr Obes | ?°Adjusting Internal Organs and Dredging Channel?± Electroacupuncture Ameliorates Insulin Resistance in Type 2 Diabetes Mellitus by Regulating the Intestinal??- [34135611] |
| Evid Based Complement Alternat Med | Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity [34659432] |
| Ecotoxicol Environ Saf | High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats [34224971] |
| Int J Food Sci Nutr | Brown and golden flaxseed reduce intestinal permeability and endotoxemia, and improve the lipid profile in perimenopausal overweight women [Pubmed:35311432] |
