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白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Interleukin 33 (IL33) ,etc. by FLIA (Flow Luminescence Immunoassay)

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

（注：單次混測多因子不超過8個指標 ）

  • 白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-92 88
EDTA plasma(n=5) 95-104 101
heparin plasma(n=5) 85-99 90

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 90-98% 89-96% 96-103%
EDTA plasma(n=5) 78-98% 89-99% 93-101% 98-105%
heparin plasma(n=5) 80-99% 93-102% 94-105% 95-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPB980Mu02 白介素33(IL33)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB980Mu01 白介素33(IL33)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB980Mu01 白介素33(IL33)多克隆抗體 WB; IHC; ICC; IP.
PAB980Mu02 白介素33(IL33)多克隆抗體 WB; IHC; ICC; IP.
LAB980Mu81 白介素33(IL33)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAB980Mu71 白介素33(IL33)多克隆抗體(生物素標記) WB; IHC; ICC.
MEB980Mu 白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB980Mu 白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB980Mu 白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB980Mu 白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
The Korean Journal of Internal Medicine Interleukin-33, matrix metalloproteinase-9, and tissue ?nhibitor of matrix metalloproteinase-1 in myocardial infarction [PubMed: PMC3604606]
Acta Histochem CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice [PubMed: 26456520]
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Journal of Interferon & Cytokine Research The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. [pubmed:27348082]
Archives of Dermatological Research IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo [pubmed:27388717]
Sci Pharm. Chrysin Protects?Rat?Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. [pubmed:28134775]
Biotechnology & Biotechnological Equipment? Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) [10.1080:13102818.2018.1471361]
Tropical Journal of Pharmaceutical Research Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer [172341]
Particle and?Fibre?Toxicology Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure [Pubmed: 30413212]
Scientific Reports Interleukin-33 serum levels in postmenopausal women with osteoporosis [Pubmed: 30846811]
Cell Reports Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s [Pubmed: 32101749]
Allergy Asthma & Immunology Research Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics [Pubmed: 32141256]
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … []
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633]
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005]
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling []
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