D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for D-Amino Acid Oxidase (DAO) ,etc. by FLIA (Flow Luminescence Immunoassay)
DAMOX
(注:單次混測多因子不超過8個指標(biāo) )
- 編號LMJ298Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.1-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.033 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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特異性
本試劑盒用于檢測D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測定并計(jì)算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 78-101 | 92 |
EDTA plasma(n=5) | 91-99 | 94 |
heparin plasma(n=5) | 91-99 | 96 |
sodium citrate plasma(n=5) | 81-91 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 94-105% | 79-95% | 95-103% | 86-98% |
EDTA plasma(n=5) | 88-103% | 78-101% | 78-104% | 88-99% |
heparin plasma(n=5) | 84-95% | 87-101% | 88-97% | 80-89% |
sodium citrate plasma(n=5) | 88-104% | 84-105% | 80-101% | 78-103% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品、樣本、磁珠)標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL,
37°C酶標(biāo)板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標(biāo)板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠,其中的D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPJ298Hu01 | D-氨基酸氧化酶(DAO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ298Hu01 | D-氨基酸氧化酶(DAO)多克隆抗體 | WB; IHC; ICC; IP. |
MAJ298Hu21 | D-氨基酸氧化酶(DAO)單克隆抗體 | WB; IHC; ICC; IP. |
SEJ298Hu | D-氨基酸氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCJ298Hu | D-氨基酸氧化酶(DAO)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMJ298Hu | D-氨基酸氧化酶(DAO)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
無 | Differential regulation of D-amino acid oxidase (DAO) mRNA and protein expression in human post-mortem brain [Dam: Jcr:3107E495-Ef68-4692-85Be-D43D9F3F84Ff] |
不確定雜志名 | Age span expression of schizophrenia susceptibility genes D-amino acid oxidase (DAO) and DAO activator (DAOA) in human post-mortem brain [dam:jcr:c3394762-a692-4fbd-8a37-7da14f429996] |
Frontiers in Neuroanatomy | Expression of D-Amino Acid Oxidase (DAO/DAAO) and D-Amino Acid Oxidase Activator (DAOA/G72) during Development and Aging in the Human Post-mortem Brain. [pubmed:28428746] |
BMC?gastroenterology | Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control study [Pubmed: 30400824] |
Journal of Gastroenterology | Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and mice [Pubmed: 30519748] |
Phytotherapy?Research | Effect of a chamomile extract in protecting against radiation‐induced intestinal mucositis [Pubmed: 30632234] |
Biological & Pharmaceutical Bulletin | Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis [Pubmed: 31582652] |
Int Immunopharmacol | Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response [Pubmed: 32361189] |
european journal of clinical nutrition? | Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury [33514869] |
Nutrients | The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP [33801020] |
J Cell Mol Med | The |á2AR/Caveolin?\1/p38MAPK/NF?\|êB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia?\reperfusion [34114328] |
BMC Gastroenterol | Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity [34022802] |
Nutrients | Psychological and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome Undergoing a Low-FODMAP Diet: The Role of the Intestinal Barrier [34371976] |
Int J Neuropsychopharmacol | Blood D-amino acid oxidase (DAO) levels increased with cognitive decline among people with mild cognitive impairment (MCI): a two-year prospective study [Pubmed:35430632] |