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親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)

High Sensitive ELISA Kit for Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

  • 親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) 產品包裝(模擬)
  • 親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) 產品包裝(模擬)
  • 親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) 實驗結果圖
  • HEA979Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-92 88
EDTA plasma(n=5) 85-101 95
heparin plasma(n=5) 89-102 98

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 87-98% 97-105% 80-101%
EDTA plasma(n=5) 88-96% 79-98% 92-105% 79-101%
heparin plasma(n=5) 95-102% 84-104% 95-102% 98-105%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。

實驗原理

將親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結合，然后加入生物素化的親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的親環(huán)素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法,高敏型)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

相關產品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
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LMA979Mu 親環(huán)素A(CYPA)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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KSA979Mu01 親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Atherosclerosis EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [PubMed: 19758589]
Biomarkers Cyclophilin-A: a novel biomarker for untreated male essential hypertension [Pubmed: 24164594]
Cardiovascular Diabetology Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease [Pubmed:24502618]
International Journal of Biological Sciences Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction [Pubmed:25552928]
Medicine Cyclophilin A in Ruptured Intracranial Aneurysm [PubMed: 26426668]
Mol Cell Biochem PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease [PubMed: 26702934]
Medicine (Baltimore) Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus [PubMed: 26496315]
International Journal of Cardiology Cyclophilin?A?modulates?bone?marrow-derived?CD117(+)?cells?and?enhances?ischemia-induced?angiogenesisvia?the?SDF-1/CXCR4?axis. [Pubmed:27057951]
Clinica?Chimica?Acta Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor [pubmed:27823952]
Cardiovascular?Diabetology Cyclophilin?A?enhances?macrophage?differentiation?and?lipid?uptake?in?high?glucose?conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. [pubmed:27809851]
Clinical?Science Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity [Pubmed:29382697]
Journal of Molecular Histology Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis [Pubmed:29318411]
Free?Radical?Research Cyclophilin A and CD147 associate with progression of diabetic nephropathy [Pubmed: 30572748]
The Kaohsiung Journal of Medical Sciences Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction [Pubmed: 31670477]
Cells. Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients [Pubmed: 31936351]
JAMA Neurology APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers?… [Pubmed: 24030206]
Menoufia Medical Journal Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy []
Toxins Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430]
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death [Pubmed:35732625]
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