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Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Toll Like Receptor 2 (TLR2)

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

  • Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SEA663Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測Toll樣受體2(TLR2)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將Toll樣受體2(TLR2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的Toll樣受體2(TLR2)與連接于固相載體上的抗體結合，然后加入生物素化的Toll樣受體2(TLR2)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的Toll樣受體2(TLR2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

相關產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPA663Ra01 Toll樣受體2(TLR2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA663Ra01 Toll樣受體2(TLR2)多克隆抗體 WB; IHC; ICC; IP.
MAA663Ra21 Toll樣受體2(TLR2)單克隆抗體 WB; IHC; ICC; IP.
SEA663Ra Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA663Ra Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
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