白介素33(IL33)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Interleukin 33 (IL33)
DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules
- 編號SEB980Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.4pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測白介素33(IL33)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素33(IL33)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-103 | 95 |
| EDTA plasma(n=5) | 92-104 | 101 |
| heparin plasma(n=5) | 78-90 | 85 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的白介素33(IL33)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素33(IL33)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-105% | 88-101% | 98-105% | 83-99% |
| EDTA plasma(n=5) | 86-98% | 92-103% | 82-97% | 96-103% |
| heparin plasma(n=5) | 96-104% | 92-105% | 97-105% | 83-101% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將白介素33(IL33)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的白介素33(IL33)與連接于固相載體上的抗體結合，然后加入生物素化的白介素33(IL33)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的白介素33(IL33)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB980Hu02 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB980Hu01 | 白介素33(IL33)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB980Hu01 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB980Hu02 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB980Hu01 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB980Hu71 | 白介素33(IL33)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| RAB980Hu21 | 白介素33(IL33)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB980Hu24 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB980Hu26 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB980Hu21 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB980Hu22 | 白介素33(IL33)單克隆抗體 | WB; IHC |
| MAB980Hu23 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB980Hu25 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| LAB980Hu72 | 白介素33(IL33)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB980Hu76 | 白介素33(IL33)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEB980Hu | 白介素33(IL33)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB980Hu | 白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB980Hu | 白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSB980Hu01 | 白介素33(IL33)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB980Hu01 | 白介素33(IL33)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| The Korean Journal of Internal Medicine | Interleukin-33, matrix metalloproteinase-9, and tissue ?nhibitor of matrix metalloproteinase-1 in myocardial infarction [PubMed: PMC3604606] |
| Acta Histochem | CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice [PubMed: 26456520] |
| Journal of Interferon & Cytokine Research | Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis [Pubmed:26540312] |
| Journal of Interferon & Cytokine Research | The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. [pubmed:27348082] |
| Archives of Dermatological Research | IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo [pubmed:27388717] |
| Sci Pharm. | Chrysin Protects?Rat?Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. [pubmed:28134775] |
| Biotechnology & Biotechnological Equipment? | Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) [10.1080:13102818.2018.1471361] |
| Tropical Journal of Pharmaceutical Research | Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer [172341] |
| Particle and?Fibre?Toxicology | Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure [Pubmed: 30413212] |
| Scientific Reports | Interleukin-33 serum levels in postmenopausal women with osteoporosis [Pubmed: 30846811] |
| Cell Reports | Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s [Pubmed: 32101749] |
| Allergy Asthma & Immunology Research | Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics [Pubmed: 32141256] |
| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … [] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633] |
| Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005] |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling [] |
