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核糖核酸酶A3(RNASE3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Ribonuclease A3 (RNASE3)

Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease,RNase A Family 3; Eosinophil Cationic Protein

  • 核糖核酸酶A3(RNASE3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 核糖核酸酶A3(RNASE3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 核糖核酸酶A3(RNASE3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品圖片
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)核糖核酸酶A3(RNASE3)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB758Hu03 核糖核酸酶A3(RNASE3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of American Science Assessment of the Role of Serum and Urine Eosinophil Cationic Protein in Diagnosis of Wuchereria bancrofti Infection [Jofamerican: source]
Molecular Medicine Reports Intercellular adhesion molecule?1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps [Pubmed:25573100]
International Journal of Molecular Sciences The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study [Pubmed:26927088]
annals of allergy asthma & immunology Interleukin 16 and CCL17/thymus and activation-regulated chemokine in patients with aspirin-exacerbated respiratory disease. [pubmed:27986411]
European?Journal?of?Clinical?Investigation Dying blood mononuclear cell secretome exerts antimicrobial activity. [pubmed:27513763]
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis []
LARYNGOSCOPE Therapeutic Effects of Intranasal Tofacitinib on Chronic Rhinosinusitis with Nasal Polyps in Mice [Pubmed: 33014124]
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis [Pubmed: 32966985]
Immunopharmacol Immunotoxicol sCD48 is elevated in non-allergic but not in allergic persistent rhinitis [34477021]
Mediators Inflamm Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps [Pubmed:35075349]
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