基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10)
STMY2; SL2; Stromelysin 2; Transin-2
- 編號KSA098Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍78.12-5000pg/mL
- 可適靈敏度34pg/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對基質(zhì)金屬蛋白酶10(MMP10)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA098Hu01 | 基質(zhì)金屬蛋白酶10(MMP10)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA098Hu01 | 基質(zhì)金屬蛋白酶10(MMP10)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA098Hu71 | 基質(zhì)金屬蛋白酶10(MMP10)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA098Hu81 | 基質(zhì)金屬蛋白酶10(MMP10)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA098Hu22 | 基質(zhì)金屬蛋白酶10(MMP10)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA098Hu | 基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA098Hu | 基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA098Hu | 基質(zhì)金屬蛋白酶10(MMP10)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA098Hu01 | 基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Cellular and Molecular Medicine | Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling [Wiley: source] |
| PLoS One. | Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential [PubMed: PMC3335833] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Brain, Behavior, and Immunity | Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model [Pubmed:25266150] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
| Brain, Behavior, and Immunity | Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model [PubMed: 25266150] |
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