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白介素15(IL15)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Interleukin 15 (IL15)

  • 白介素15(IL15)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 白介素15(IL15)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對白介素15(IL15)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA061Mu02 白介素15(IL15)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAA061Mu01 白介素15(IL15)多克隆抗體 WB; IHC; ICC; IP.
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LAA061Mu71 白介素15(IL15)多克隆抗體(生物素標記) WB; IHC; ICC.
LAA061Mu81 白介素15(IL15)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
SEA061Mu 白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA061Mu 白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA061Mu 白介素15(IL15)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA061Mu01 白介素15(IL15)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180]
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723]
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886]
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle]
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice [s10529-017-2342-1]
Journal?of?Controlled?Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. [pubmed:28408199]
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