腫瘤壞死因子受體超家族成員9(TNFRSF9)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9)
CD137; CDw137; 4-1BB; ILA; Induced By Lymphocyte Activation; T-cell antigen 4-1BB homolog
- 編號APB527Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點8.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
TNFRSF9 (Tumor necrosis factor receptor superfamily member 9) is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. A binding ELISA assay was conducted to detect the interaction of TNFRSF9 and TNFa. Briefly, recombinant human TNFRSF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF9 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB527Hu01 | 腫瘤壞死因子受體超家族成員9(TNFRSF9)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB527Hu01 | 腫瘤壞死因子受體超家族成員9(TNFRSF9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB527Hu01 | 腫瘤壞死因子受體超家族成員9(TNFRSF9)多克隆抗體 | WB;FCM |
| FAB527Hu01 | 抗腫瘤壞死因子受體超家族成員9(TNFRSF9)多克隆抗體 | Flow cytometry. |
| LAB527Hu71 | 腫瘤壞死因子受體超家族成員9(TNFRSF9)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| RAB527Hu21 | 腫瘤壞死因子受體超家族成員9(TNFRSF9)重組抗體 | WB; IFC; IHC; IP. |
| SEB527Hu | 腫瘤壞死因子受體超家族成員9(TNFRSF9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB527Hu | 腫瘤壞死因子受體超家族成員9(TNFRSF9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
