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轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白

Active Transferrin Receptor (TFR)

CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

  • 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 產(chǎn)品包裝(模擬)
  • 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 產(chǎn)品包裝(模擬)
  • APB171Hu62.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Transferrin Receptor (TFR) , also known as cluster of differentiation 71 (CD71), is a type II transmembrane glycoprotein that plays a crucial role in the transport of iron into the cell. It binds to Transferrin (TRF), a carrier protein that transports iron in the blood, and facilitates the uptake of iron through endocytosis. This process is essential for many biological functions, including DNA synthesis and cell growth.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TFR and recombinant chicken TRF. Briefly, biotin-linked TFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TRF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of TFR and TRF was shown in Figure 1, the EC50?for this effect is 0.068ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
APB171Hu62 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 Cell?culture;?Activity?Assays.
APB171Hu61 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 Cell?culture;?Activity?Assays.
EPB171Hu61 轉(zhuǎn)鐵蛋白受體(TFR)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB171Hu01 轉(zhuǎn)鐵蛋白受體(TFR)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB171Hu62 轉(zhuǎn)鐵蛋白受體(TFR)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB171Hu01 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體 WB; IHC
CAB171Hu01 抗轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體 Loading Control of WB.
LAB171Hu81 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAB171Hu71 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體(生物素標記) WB; IHC; ICC.
CAB171Hu24 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 Loading Control of WB.
MAB171Hu22 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 IHC
MAB171Hu26 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 WB
MAB171Hu23 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 WB; IHC; ICC; IP.
MAB171Hu24 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 WB; IHC; ICC; IP.
MAB171Hu25 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 WB
MAB171Hu21 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 WB; IHC; ICC; IP.
CAB171Hu22 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 Loading Control of WB.
FAB171Hu02 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 Flow cytometry.
FAB171Hu86 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體(異硫氰酸熒光素標記) FCM
FAB171Hu42 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體(藻紅素標記) FCM
SEB171Hu 轉(zhuǎn)鐵蛋白受體(TFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB171Hu 轉(zhuǎn)鐵蛋白受體(TFR)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
British Journal of Nutrition Survey of anaemia and Helicobacter pylori infection in adolescent girls in Suihua, China and enhancement of iron intervention effects by H. pylori eradication [PubMed: 22004585]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743]
Clinical and Experimental?Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502]
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
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