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腦啡肽酶(CD10)活性蛋白

Active Neprilysin (CD10)

NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase

  • 腦啡肽酶(CD10)活性蛋白 產(chǎn)品包裝(模擬)
  • 腦啡肽酶(CD10)活性蛋白 產(chǎn)品包裝(模擬)
  • APB785Hu61.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Neprilysin/CD10, also known as NEP and neutral endopeptidase, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide. NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states. The activity of recombinant human NEP is measured by its ability to cleave a fluorogenic peptide substrate MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH in the assay buffer 50 mM Tris, 0.05% Brij-35, pH 9.0. The rhNEP is diluted to 0.1 ug/ml in assay buffer, then add 50 μL protein into a black well plate and start the reaction by adding 50 μL of 20 μM substrate (diluted by assay buffer), with a substrate blank containing 50 μL assay buffer, 50 μL substrate, and no rhNEP. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human NEP is > 3500 pmol/min/μg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APB785Hu61 腦啡肽酶(CD10)活性蛋白 Cell?culture;?Activity?Assays.
RPB785Hu02 腦啡肽酶(CD10)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB785Hu01 腦啡肽酶(CD10)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB785Hu61 腦啡肽酶(CD10)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB785Hu01 腦啡肽酶(CD10)多克隆抗體 WB; IHC; ICC; IP.
PAB785Hu02 腦啡肽酶(CD10)多克隆抗體 WB; IHC; ICC; IP.
LAB785Hu71 腦啡肽酶(CD10)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB785Hu21 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu23 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu24 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu22 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu25 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu27 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu29 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
MAB785Hu26 腦啡肽酶(CD10)單克隆抗體 WB; IHC; ICC; IP.
FAB785Hu02 抗腦啡肽酶(CD10)單克隆抗體 FCM
FAB785Hu04 抗腦啡肽酶(CD10)單克隆抗體 FCM
FAB785Hu06 抗腦啡肽酶(CD10)單克隆抗體 FCM
FAB785Hu08 抗腦啡肽酶(CD10)單克隆抗體 FCM
FAB785Hu00 抗腦啡肽酶(CD10)單克隆抗體 Flow cytometry.
FAB785Hu82 抗腦啡肽酶(CD10)單克隆抗體(異硫氰酸熒光素標(biāo)記) FCM
SEB785Hu 腦啡肽酶(CD10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB785Hu 腦啡肽酶(CD10)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
13 Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors [Ahajournals:Source]
Biol Pharm Bull Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats [PubMed: 25832640]
Eur J Heart Fail.? Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction [Pubmed:26725876]
8 A?Test?in?Context:?Neprilysin:?Function,?Inhibition, and?Biomarker. [pubmed:27491909]
European?Journal of?Heart?Failure Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study [Pubmed: 30520545]
European?Journal of?Heart?Failure Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure [Pubmed: 30828920]
Clinical Research in Cardiology Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) [Pubmed: 32002632]
Neurotoxicology Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice [Pubmed: 32522471]
European Heart Journal P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction []
Clin Biochem Finding a reliable assay for soluble neprilysin [Pubmed:35331754]
Scientific Reports The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in?… []
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