單核細胞趨化蛋白4(MCP4)活性蛋白
Active Monocyte Chemotactic Protein 4 (MCP4)
CCL13; SCYA13; NCC1; SCYL1; CKb10; Chemokine C-C-Motif Ligand 13; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13; CK-beta-10; Monocyte chemoattractant 4
- 編號APA216Hu51
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點10.0
- 應用Cell?culture;?Activity?Assays;?In?vivo?assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant MCP4, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
CCL13 (C-C motif chemokine 13) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, which belongs to the CC chemokine subfamily. It has been reported that CCL13 can induce chemotactic migration of THP-1 cells. Therefore, chemotaxis?assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CCL13 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100μL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and CCL13 (50ng/mL and 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
Result: CCL13 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen to count the migrated cells at high magnification (×400) and the statistical data was shown in Figure 2.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPA216Hu62 | 單核細胞趨化蛋白4(MCP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA216Hu01 | 單核細胞趨化蛋白4(MCP4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA216Hu51 | 單核細胞趨化蛋白4(MCP4)活性蛋白 | Cell?culture;?Activity?Assays;?In?vivo?assays. |
| RPA216Hu02 | 單核細胞趨化蛋白4(MCP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA216Hu51 | 單核細胞趨化蛋白4(MCP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA216Hu01 | 單核細胞趨化蛋白4(MCP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA216Hu61 | 單核細胞趨化蛋白4(MCP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA216Hu07 | 單核細胞趨化蛋白4(MCP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA216Hu02 | 單核細胞趨化蛋白4(MCP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA216Hu51 | 單核細胞趨化蛋白4(MCP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA216Hu01 | 單核細胞趨化蛋白4(MCP4)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA216Hu22 | 單核細胞趨化蛋白4(MCP4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA216Hu | 單核細胞趨化蛋白4(MCP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA216Hu | 單核細胞趨化蛋白4(MCP4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
