CD7分子(CD7)活性蛋白
Active Cluster Of Differentiation 7 (CD7)
GP40; LEU9; TP41; Tp40; p41 Protein; T-cell Antigen CD7; T-cell Leukemia Antigen; T-cell surface antigen Leu-9
- 編號APB784Hu61
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點4.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
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活性實驗
Cluster Of Differentiation 7 (CD7)?is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. SECTM1?has been proposed as a ligand for?CD7. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD7 and recombinant human SECTM1. Briefly, biotin-linked CD7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SECTM1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of CD7 and SECTM1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB784Hu01 | CD7分子(CD7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB784Hu61 | CD7分子(CD7)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB784Hu02 | CD7分子(CD7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB784Hu61 | CD7分子(CD7)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB784Hu01 | CD7分子(CD7)多克隆抗體 | WB; IHC; ICC; IP. |
MAB784Hu22 | CD7分子(CD7)單克隆抗體 | WB; IHC; ICC; IP. |
MAB784Hu25 | CD7分子(CD7)單克隆抗體 | WB; IHC; ICC; IP. |
MAB784Hu21 | CD7分子(CD7)單克隆抗體 | WB; IHC; ICC; IP. |
MAB784Hu23 | CD7分子(CD7)單克隆抗體 | WB; IHC; ICC; IP. |
MAB784Hu24 | CD7分子(CD7)單克隆抗體 | WB. |