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載脂蛋白C1(APOC1)活性蛋白

Active Apolipoprotein C1 (APOC1)

Apo-C1; Truncated apolipoprotein C-I

  • 載脂蛋白C1(APOC1)活性蛋白 產品包裝(模擬)
  • 載脂蛋白C1(APOC1)活性蛋白 產品包裝(模擬)
  • APA252Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Apolipoprotein C1 (APOC1), also known as?Apo-CI; ApoC-I;?apo-CIB;?apoC-IB,?is the smallest size apolipoprotein of all apolipoprotein C family (Mr = 6.6 kDa) and located at position 19q13.32. APOC1 is primarily expressed in the liver and activated when monocytes differentiate into macrophages. It plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. It has anti-inflammatory activity and also can promote the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Besides, Apolipoprotein C3 (APOC3) has been identified as an interactor of APOC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOC1 and recombinant mouse APOC3. Briefly, APOC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOC3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APOC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human APOC1 and recombinant mouse APOC3 was shown in Figure 1, the EC50?for this effect is 2.19 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
RPA252Hu01 載脂蛋白C1(APOC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA252Hu01 載脂蛋白C1(APOC1)活性蛋白 Cell?culture;?Activity?Assays.
APA252Hu02 載脂蛋白C1(APOC1)活性蛋白 Cell?culture;?Activity?Assays.
RPA252Hu02 載脂蛋白C1(APOC1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA252Hu01 載脂蛋白C1(APOC1)多克隆抗體 WB; IHC; ICC/IF; FCM
PAA252Hu02 載脂蛋白C1(APOC1)多克隆抗體 WB; IHC; ICC; IP.
LAA252Hu71 載脂蛋白C1(APOC1)多克隆抗體(生物素標記) WB; IHC; ICC.
MAA252Hu21 載脂蛋白C1(APOC1)單克隆抗體 WB
MAA252Hu23 載脂蛋白C1(APOC1)單克隆抗體 WB; IHC; ICC; IP.
MAA252Hu22 載脂蛋白C1(APOC1)單克隆抗體 WB; IHC; ICC; IP.
FAA252Hu02 抗載脂蛋白C1(APOC1)單克隆抗體 FCM
FAA252Hu04 抗載脂蛋白C1(APOC1)單克隆抗體 Flow cytometry.
SEA252Hu 載脂蛋白C1(APOC1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA252Hu 載脂蛋白C1(APOC1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
CGA252Hu02 載脂蛋白C1(APOC1)cDNA PCR、蛋白表達等

參考文獻

雜志 參考文獻
PROTEOMICS - Clinical Applications Proteomic and genomic analyses suggest the association of Apolipoprotein C1 with abdominal aortic aneurysm [Onlinelibrary: prca.201300119]
Journal of Dairy Science Colostrum and milk protein rankings and ratios of importance to neonatal calf health using a proteomics approach [pubmed:28189329]
RESPIRATORY RESEARCH The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation?… [Pubmed: 33148285]
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