酸性磷酸酶1(ACP1)活性蛋白
Active Acid Phosphatase 1 (ACP1)
HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase
- 編號(hào)APA994Mu02
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1056 ¥ 2640 ¥ 5280 ¥ 15840 ¥ 39600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl?phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 ( assay buffer), initiated by addition 50 μl of 3 ug/ml ACP1 (diluted by assay buffer) to 50 μL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 μl of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA994Mu01 | 酸性磷酸酶1(ACP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA994Mu02 | 酸性磷酸酶1(ACP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA994Mu02 | 酸性磷酸酶1(ACP1)活性蛋白 | Cell?culture;?Activity?Assays. |
