酸性磷酸酶1(ACP1)活性蛋白
Active Acid Phosphatase 1 (ACP1)
HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase
- 編號APA994Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. EFNA1 is a kind of receptor tyrosine kinase which can Interact with ACP1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA1 and recombinant human ACP1. Briefly, EFNA1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACP1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EFNA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. When recombinant human ACP1 is immobilized at 2 ug/ml (100 ul/well), the concentration of EFNA1 that produces 50% optimal binding response is found to be approximately 0.21 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA994Hu02 | 酸性磷酸酶1(ACP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA994Hu01 | 酸性磷酸酶1(ACP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA994Hu01 | 酸性磷酸酶1(ACP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA994Hu02 | 酸性磷酸酶1(ACP1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA994Hu01 | 酸性磷酸酶1(ACP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA994Hu81 | 酸性磷酸酶1(ACP1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA994Hu71 | 酸性磷酸酶1(ACP1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA994Hu21 | 酸性磷酸酶1(ACP1)單克隆抗體 | WB |
| MAA994Hu22 | 酸性磷酸酶1(ACP1)單克隆抗體 | WB; IHC; ICC; IP. |
| KSA994Hu01 | 酸性磷酸酶1(ACP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
