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腎足蛋白(NPHN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

  • 腎足蛋白(NPHN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 腎足蛋白(NPHN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)腎足蛋白(NPHN)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA937Ra01 腎足蛋白(NPHN)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA937Ra01 腎足蛋白(NPHN)多克隆抗體 WB; IHC; ICC; IP.
MAA937Ra21 腎足蛋白(NPHN)單克隆抗體 WB; IHC; ICC; IP.
SEA937Ra 腎足蛋白(NPHN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA937Ra 腎足蛋白(NPHN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA937Ra01 腎足蛋白(NPHN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Yonsei medical journal The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. [pubmed:28120572]
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788]
British Journal of Haematology? Renal protection by atorvastatin in a murine model of sickle cell nephropathy [Pubmed:29527679]
Journal of Clinical Medicine Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease [Pubmed: 32961903]
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study [34707724]
Biomed Rep Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs [Pubmed:35620307]
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